Tsc2 cst

WebThe TSC2 gene provides instructions for producing a protein called tuberin. Within cells, tuberin interacts with a protein called hamartin, which is produced from the TSC1 gene. These two proteins help control cell growth and division (proliferation) and cell size. Proteins that normally prevent cells from growing and dividing too fast or in an ... WebAug 24, 2012 · The tuberous sclerosis complex (TSC) tumor suppressors form the TSC1-TSC2 complex, which limits cell growth in response to poor growth conditions. Through …

TSC2 Gene - GeneCards TSC2 Protein TSC2 Antibody

WebFeb 23, 2024 · HDAC6 deacetylates TSC2 to sustain protein stability and associates with G3BP2 to facilitate more ... CST), Rheb (1:100; 13879, CST), G3BP2 (1:500; 16276-1-AP, … WebDec 2, 2024 · RIPK1 scaffold activity is known to have a pro-survival role in TNFα signaling. Najafov et al. discover an unexpected role for RIPK1 scaffold activity in mediating mTORC1 inhibition by AMPK during energetic stress. Mechanistic studies reveal that RIPK1 is involved in regulating TSC2 phosphorylation at Ser1387 by AMPK. Reduced phosphorylation of this … grand county co building dept https://ricardonahuat.com

RIPK1 Promotes Energy Sensing by the mTORC1 Pathway - Molecular Cell

WebApr 25, 2008 · AMPK phosphorylation of the TSC2 tumor suppressor contributes to suppression of mTORC1; however, TSC2-deficient cells remain responsive to energy stress. Using a proteomic and bioinformatics approach, we sought to identify additional substrates of AMPK that mediate its effects on growth control. WebFor immunoprecipitation of endogenous TSC and mTOR complexes, cell lysates were incubated with TSC2 antibody (CST, 4308) or mTOR antibody (CST, 2972) at 4oC with rocking for 4 hours, followed by an additional 2 hour incubation with 30µl of 50% slurry A/G-agarose beads (Thermo Scientific/Pierce, 20421). WebPolyclonal Antibody for studying Tuberin/TSC2 (Ser939) phosphate. Cited in 51 publications. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously … chinese buffet cardiff bay

(PDF) p53 Deletion or Hot-spot Mutations Enhance mTORC1

Category:TSC2 Interacts with HDLBP/Vigilin and Regulates Stress Granule ...

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Tsc2 cst

AMPK phosphorylation of raptor mediates a metabolic checkpoint

WebMay 17, 2024 · Loss-of-function mutations in TSC1 or TSC2, but not TBC1D7, give rise to TSC, a pleiotropic disorder with aberrant ... CST #5425) was used as a blocking solution … Web1.1.2 主要试剂：DMEM-高糖培养基(Corning公司)；胎牛血清和EDTA-0.25% 胰蛋白酶(Gibco公司)；噻唑蓝(MTT)和二甲基亚砜(DMSO)(Ameresco公司)；氢氧化钠(NaOH)(北京化工厂)；雷帕霉素(rapamycin，Rapa)和羟乙基哌嗪乙硫磺酸(HEPES)(Sigma-Aldrich公司)；多聚甲醛和结晶紫(武汉塞维尔公司)；细胞周期与细胞凋亡检测试剂盒 ...

Tsc2 cst

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WebSanborn. Sep 2024 - Present8 months. Colorado Springs, Colorado, United States. Responsible for Operations of the Sanborn Mapping Business Unit. This includes ortho and oblique imagery technology ... WebSep 18, 2015 · In cells with basal wt p53 function, p53-dependent transcription supplies adequate levels of TSC2 and sestrin2 to regulate mTORC1 kinase activity; when cells lose p53 transcriptional activity, TSC2 and Sestrin2 levels are low, TSC2 binding at the lysosomal sites of mTORC1 is low, Rheb levels are higher at these sites, and PRAS40 is lower in …

WebAug 1, 2024 · AbstractTuberous sclerosis complex (TSC) is caused by mutations of either the TSC1 or TSC2 tumor suppressor gene. TSC causes tumors of the brain, heart, kidney, skin and lymphangioleiomyomatosis (LAM). Here we report that the TSC2 protein physically binds to high-density lipoprotein binding protein (HDLBP), also called vigilin, a core stress … WebMar 21, 2024 · TSC2 (TSC Complex Subunit 2) is a Protein Coding gene. Diseases associated with TSC2 include Lymphangioleiomyomatosis and Focal Cortical Dysplasia, …

WebDirections for Use: CST recommends transfection with 100 nM Tuberin/TSC2 siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the … WebMay 17, 2024 · Loss-of-function mutations in TSC1 or TSC2, but not TBC1D7, give rise to TSC, a pleiotropic disorder with aberrant ... CST #5425) was used as a blocking solution for 1 h at room temperature. Primary antibodies, p(S235/236)S6 (CST #4858, 1:400), fast skeletal Myosin (Sigma #M4276, 1:400), and slow skeletal Myosin (Abcam # ...

WebGlaciecola sp. kul10 es una bacteria que se encuentra en ambientes extremos como los glaciares y los océanos. Su capacidad para producir enzimas y compuestos bioactivos la hace de interés para la investigación en campos como la …

WebMay 1, 2024 · Germline or mosaic loss-of-function mutations in either TSC1 or TSC2 give rise to the pleiotropic genetic ... 2.7 mM KCl, 0.1% Tween-20) plus 5% normal goat serum (NGS; CST #5425) was used as a blocking solution for 1 h at room temperature. Primary antibodies, p(S235/236)S6 (CST #4858, 1:400), fast skeletal Myosin (Sigma #M4276 ... chinese buffet cakeWebJun 28, 2024 · To evaluate the knockdown efficiency of siRNA-TSC2 at the level of TSC2, high glucose-cultured podocytes were transfected with three different siRNA-TSC2 sequences. After TSC2 siRNA treatment for 24 h, Western blot analysis showed that transfection of TSC2 siRNA-1, TSC2 siRNA-2, and TSC2 siRNA-3 significantly reduced the … chinese buffet cake recipeWebApr 20, 2024 · Of the five gross deletions in TSC2, three involved at least exons 40–46 of PKD1 (Fig. 3) resulting in the TSC2-PKD1 contiguous gene syndrome (CGS, MIM #600273), which were previously published 17. chinese buffet by harrisburg mallWebApr 20, 2024 · Of the five gross deletions in TSC2, three involved at least exons 40–46 of PKD1 (Fig. 3) resulting in the TSC2-PKD1 contiguous gene syndrome (CGS, MIM … grand county co clerk and recorderhttp://www.ybiotech.com.cn/ybiotech2012-Products-18645796/ grand county colorado assessor pageWebTuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 … grand county co libraryWebCell Signaling Technology (CST) allows the researcher to specifically inhibit tuberin/TSC2 expression using RNA interference, a method whereby gene expression can be selectively … chinese buffet carling avenue ottawa