WebAug 1, 1999 · A 13-kDa phosphoamidase was isolated as a single band on SDS-PAGE from bovine liver. Its Stokes' radius, sedimentation coefficient, molecular mass, and optimal pH were estimated to be 1.6 nm, 1.8 s, 13 kDa, and 6.5, respectively. WebThe present invention relates to the use of compounds and to methods for the qualitative and/or quantitative determination of the activity of phosphoamidases or protein phosphoamidases specific for hydrolyzing phosphoamide (P-N) bonds of N-phosphorylated basic amino acids like phospholysine, phosphoarginine and phosphohistidine.

Bovine Liver Phosphoamidase as a Protein Histidine/Lysine Phosphatase …

WebPhosphoamidase activity of the porcine liver phospho-histidine phosphatase was assayed by incubating the phosphatase with 1 mM phosphoamidate in 25 mM Hepes, pH 7.5, at 30 C for 30 min [15]. The released orthophos-phate was measured by a modification of the method of Martin and Doty [21], using toluene instead of benzene. To WebAlkaline phosphatase is a homodimeric protein enzyme (86 kDa), containing two zinc atoms crucial to its catalytic function per monomer, and it is optimally active at alkaline pH environments. The enzyme is found in prokaryotes and eukaryotes, with the same general function but in different structural forms suitable to organism's environment. diary block

Phosphoramidate - Wikipedia

WebSUMMARY Phosphoamidase, an enzyme that catalyzes the hydrolysis of amides of phosphoric acid, has been purified about 50-fold from an extract of beef spleen. Enzymic … WebEnzyme Commission Primary Name: phosphoamidase . Enzyme Commission Synonyms: creatine phosphatase . Standard Gibbs Free Energy (Δ r G '°): 19.397003 kcal/mol [Latendresse13] Enzyme Commission Summary: Also acts on N-phospho-arginine and other phosphoamides. WebA phosphoamidase unit ([EUIPA) is defined as the amount of enzyme that catalyzes the release of 1 pmole of NH3 per ml. of incubation mixture in 10 minutes under the above conditions. In the range 0.5 to 2 units per ml., the hydrolysis of phosphoramidate was proportional to enzyme concentration. diary blocking