Phospho erk molecular weight
WebIn rodents, for example mouse ERK1b has a smaller molecular weight than mouse ERK1 (−0.67 kD) while rat ERK1b has a larger molecular weight than rat ERK1 (+2.66 kD). ... In these cells ERK2 is much more expressed than ERK1 as determined by phospho-ERK levels (Figure 6A of Woodson and Kedes, ... WebMar 23, 2024 · p-Akt1 Antibody (5.Ser 473) is an IgG1 κ mouse monoclonal p-Akt1 antibody that detects Ser 473 phosphorylated Akt1 of mouse, rat and human origin by WB, IP and ELISA. p-Akt1 Antibody (5.Ser 473) is available as both conjugated and non-conjugated anti-p-Akt1 antibody forms.
Phospho erk molecular weight
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Web1. block for 1h @ room temperature (RT) with 5% BSA 2. 1:1000 Erk or p-Erk (cell signalling) in 5% BSA, for 1h @ RT and O/N @ 4'C 3. 1:5000 2nd Ab, for 1h @ RT How can I get two bands for... WebThe MEK phosphorylates and activates a mitogen-activated protein kinase (MAPK). RAF and MAPK/ERK are both serine/threonine-specific protein kinases. MEK is a serine/tyrosine/threonine kinase. In a technical sense, …
WebThe mitogen-activated protein (MAP) kinases ERK-1 and ERK-2 are proline-directed kinases that are activated through concomitant phosphorylation of tyrosine and threonine residues. The JNK family, which includes JNK1, JNK2 ... Molecular Weight of p-JNK p46 isoform: 46 kDa. Molecular Weight of p-JNK p54 isoform: 54 kDa. Med. 19: 849-860. WebFlow cytometric analysis of ERK1/2 (pT202/pY204). Human peripheral blood mononulcear cells (PBMC) were either left unstimulated (unshaded) or stimulated (shaded) with 40 nM …
WebMolecular weight of phospho/total AKT in western blot, non-specific binding? I am looking at expression of phospho/total AKT in mouse skeletal muscle tissue lysates of lean and obese animals,... WebWhat is the molecular weight of Erk? Nonphosphorylated p44/42 MAPK (Erk1/2) Control Protein: Bacterially expressed, kinase inactive, Erk2 protein serves as a negative control …
WebFeb 13, 2024 · Keep in mind that each phosphorylation adds 80 Da to the molecular weight of the protein ( Walsh 2006 ). 3 Probing for Total Protein Using an antibody that detects the total level of your target protein enables you to determine the phosphorylated fraction relative to the total fraction.
WebOverview Product name Anti-ERK1 (phospho T202) + ERK2 (phospho T185) antibody [EPR19401] Description Rabbit monoclonal [EPR19401] to ERK1 (phospho T202) + ERK2 (phospho T185) Host species Rabbit Tested applications Suitable for: ICC/IF, IP, IHC-P, WB, Dot blot more details Species reactivity Reacts with: Mouse, Rat, Human Immunogen ctstage7dx/ctinstallWebKeep in mind that each phosphorylation adds 80Da to the protein molecular weight (Walsh 2006). 3 Probing for the total protein Using an antibody that detects the total level of your … ctstage 6WebIsotype: Mouse IgG1 Immunogen: Phosphorylated Rat ERK1 (T202/Y204) Peptide Application: Intracellular staining (flow cytometry) (Routinely Tested) Vol. Per Test: 5 µl RRID: AB_10895978 Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide. Regulatory Status: RUO View All Formats Preparation And Storage ctstage cloudtmWebPhospho-ERK1/2 (Thr202, Tyr204) Antibody (14-9109-82) in IHC (P) Immunohistochemistry of formalin-fixed paraffin embedded human breast cancer tissue stained with 5 µg/mL of … cts tableWebInvitrogen Anti-Phospho-ERK1/ERK2 (Tyr204) Polyclonal, Catalog # PA5-36038. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Immunohistochemistry (Paraffin) … earwig nestWebNational Center for Biotechnology Information earwig night galleryWebMicrotubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Tubulin is a heterodimer, which consists of a-tubulin and b-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. earwig movie explained