Phospho eif2 alpha antibody
WebMay 11, 2024 · Human Phospho-eIF2 alpha (S52) Antibody Summary. Detects human Phospho-eIF2 alpha (S52) in ELISAs and Western blots. Lyophilized from a 0.2 μm … WebSubunit Structure: Heterotrimer composed of an alpha, a beta and a gamma chain. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. …
Phospho eif2 alpha antibody
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WebJun 8, 2024 · The phosphorylation of eIF2α occurs on serine 51 in response to the activation of one out of four eIF2α kinases 4, 5, 6. EIF2AK1, commonly known as heme-regulated … WebEIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa. Protocols Publications All (7) KD/KO (1) WB (7) IP (2)
WebPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser51 of human eIF2alpha. Antibodies are purified by protein A and peptide affinity chromatography. Validations None provided Applications Western Blotting (WB) Reactivity Chlorocebus sabaeus (Green monkey) WebWestern blot - Phospho-eIF2α-S51 Rabbit pAb (AP0342) Western blot analysis of extracts of various cell lines, using Phospho-eIF2α-S51 antibody (AP0342) at 1:1000 dilution.HeLa and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014 ...
WebEIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of …
WebThis clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells. This recombinant antibody clone is derived from clone A18028A. This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and mouse (Tyr847) orthologs due to complete ...
WebFeb 26, 2024 · Anti-EIF2S1 (phospho S51) (eIF2-alpha) antibody. Catalog Number: ab32157. Info:Supplier Page View Product Specs View Product. Browse Similar Products: EIF2S1 Western Blot Antibodies. This antibody … the prom girlWebEIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; … the prom foxWebWestern Blot. Detection of Human Phospho-eIF2 alpha (S52) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 100nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes or 20 … signature ideas for the letter aWebEIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; … the prom filmWebNov 26, 2024 · Phospho-eIF2α has increased affinity to the eIF2β subunit and prevents the regeneration of guanosine triphosphate (GTP) in the ternary complex eIF2-GTP-Met tRNAi, thus halting the initiation of ... signature icon sherpa full-zip hoodieWebIHC-P analysis of human breast carcinoma tissue using GTX50300 eIF2 alpha (phospho Ser51) antibody. GTX50300 WB Image WB analysis of K562 cells untreated or treated with IFN-α (100ng/ml, 20min) lysates using GTX50300 eIF2 alpha (phospho Ser51) antibody (Lane 3 and 4) and eIF2α antibody (Lane 1 and 2). ... the prom film 2020 castWebProduct Name eIF2 alpha (Phospho-Ser51) Rabbit mAb; Host Species Rabbit; Clonality Monoclonal; Isotype Rabbit IgG; Purification Affinity-chromatography; Applications WB … signature incorporated