Phis1522
Webb6 nov. 2008 · Results: The toxin genes tcdA and tcdB were amplified by PCR using chromosomal DNA from a toxigenic strain as a template, and cloned into a shuttle vector … WebbEventually, mutations were introduced in pHIS1522/TcdB by site-directed mutagenesis (QuikChange, Stratagene, La Jolla, CA, USA). Cloning of TcdB constructs with internal deletions was performed in the pHIS1522 vector backbone by first introducing a silent SpeI restriction site at bp position 2542 of the TcdB sequence with the QuikChange method.
Phis1522
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WebbIf you already have clones is phis1522 for b.megateriun you can buy the brevibacillus cells from takara and test it its quite simple and fast. WebbJuly 2, 2015: An aliquot of purified pHis1522-aTcdB vector was acquired from Dr. Xingmin Sun from the Tufts Vet School. NIH-recommended simple letter format MTA forms were signed by both parties. (aTcdB = atoxic Clostridium difficile toxin B) July 6, 2015: The pHis1522-aTcdB was diluted 1:10 and 1ul was transformed into JM109 E. coli.
WebbHuge Selection of Cloning and Expression Vectors. MoBiTec is leading in the development of unique and cutting edge vector systems for recombinant protein expression. We offer … Webb21 mars 2016 · Size-reduced pHIS1522 variant with sequence for C-terminal StrepII-tag fusion (including stop codon directly downstream of the tag). pN-His-TEV1622 Like …
WebbSize-reduced pHIS1522 variant with sequence for C-terminal 6xHis-tag fusion (includingstop codon right downstream of the tag). WebbpC-Strep1622 (BMEG21)-size-reduced pHIS1522 variant with sequence for C-terminal StrepII-tag fusion (including stop codon directly downstream of the tag). Vectors of the …
Webb6 nov. 2008 · Background Major Clostridium difficile virulence factors are the exotoxins TcdA and TcdB. Due to the large size and poor stability of the proteins, the active recombinant TcdA and TcdB have been difficult to produce. Results The toxin genes tcdA and tcdB were amplified by PCR using chromosomal DNA from a toxigenic strain as a …
WebbFor production of target proteins without any tag plasmids for intracellular production (pWH1520, pMM1522, pSTOP1622) and extracellular production (pMM1525) are … can an 8 year old be charged with assaultWebbPRODUCT NOTES. Replaces old part number 3867561. This is a Cummins Blowby Flow Sensor for KTA, QSK, QST and QSV engines. This is a genuine Cummins part shipped … can an 8 month old boy dog make puppiesWebbpHIS1522 pSTOP1522 derivative – vector for intracellular production of His6-tagged proteins in B. megaterium this work pSTREP1525 Vector for the secretion of … fishers hamiltonWebbpHIS1522 (MoBiTec), the gene was amplified by PCR from genomic DNA (C.novyi strain 6018) in two parts. First, the upstream sequence of an intrinsic SpeI restriction site at bp 2965 was amplified with oligonucleotides introducing a 5-BsrGIrestrictionsite,andsecond,thedownstreamsequence of the SpeI restriction site was … can an 8 year old take pepto bismolWebbTaKaRa brevibacillus Brevibacillus, supplied by TaKaRa, used in various techniques. Bioz Stars score: 80/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more fishers hamilton libraryWebbPlasmid Phis1522, supplied by Mobitec, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol … fishers happy hour northportWebbTaKaRa brevibacillus competent cells takara bio Brevibacillus Competent Cells Takara Bio, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. fishers ham and meat